Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Efficient mapping of protein antigenic determinants.

V Mehra, D Sweetser, R A Young

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Management strategies following recurrence of resected retroperitoneal sarcoma: A 10-year observational study.

    European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2025
    Same author

    Human parainfluenza virus type 3 infections in a haemato-oncology unit: social distancing measures needed in outpatient clinics.

    The Journal of hospital infection·2021
    Same author

    APOBEC signature mutation generates an oncogenic enhancer that drives LMO1 expression in T-ALL.

    Leukemia·2017
    Same author

    Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer.

    Oncogene·2016
    Same author

    TRIB2 reinforces the oncogenic transcriptional program controlled by the TAL1 complex in T-cell acute lymphoblastic leukemia.

    Leukemia·2015
    Same author

    The lived experience of visual creative expression for young adult cancer survivors.

    European journal of cancer care·2014
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Researchers identified six unique antigenic determinants on the Mycobacterium leprae 65-kDa antigen using recombinant DNA technology. This method rapidly elucidates protein epitopes for potential diagnostic and therapeutic applications in leprosy.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Microbiology

    Background:

    • Leprosy, caused by Mycobacterium leprae, presents diagnostic challenges.
    • Understanding the antigenic determinants of M. leprae is crucial for developing diagnostics and vaccines.

    Purpose of the Study:

    • To deduce the amino acid sequences of six antigenic determinants within the M. leprae 65-kDa antigen.
    • To establish a rapid method for epitope elucidation using recombinant DNA technology.

    Main Methods:

    • Sequencing the M. leprae 65-kDa antigen gene.
    • Constructing a lambda gt11 gene sublibrary.
    • Screening recombinant clones with monoclonal antibodies.
    • Determining DNA insert sequences via primer-extension.
    • Deducing amino acid sequences from DNA overlaps.

    Related Experiment Videos

    Main Results:

    • Successfully elucidated the amino acid sequences of six distinct epitopes.
    • Synthesized a peptide containing one epitope, which bound the specific monoclonal antibody.
    • Confirmed one antigenic determinant as unique to M. leprae.

    Conclusions:

    • The recombinant DNA strategy provides a rapid and effective method for epitope mapping.
    • This approach can be applied to identify epitopes recognized by antibodies or T cells.
    • The identified epitopes may serve as targets for future leprosy diagnostics or therapeutics.