Related Experiment Video
Updated: May 5, 2026

09:08
Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
Published on: November 4, 2025
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Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian
Aoi Teraoka1, Kanako Murakoshi, Kyoko Fukamauchi
1Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan. furuta@biomol.sci.toho-u.ac.jp.
Abstract:
A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.

