Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergic Effect of Enzyme-Assisted Extraction and Natural Deep Eutectic Solvents for Bioactives Recovery in Orange Peel Waste.

Biotechnology and applied biochemistry·2026
Same author

Structural Basis for Trivalent Cross-Linking of a Patient-Derived IgE Antibody by the Major Peanut Allergen Ara h 2.0201.

Allergy·2026
Same author

Gold Nanoparticles as a Possible Tool to Untangle Some Structural Features of the Gluten Network.

Foods (Basel, Switzerland)·2025
Same author

Exploring Germination to Unlock the Nutritional Potential of Sorghum (<i>Sorghum bicolor</i>).

Molecules (Basel, Switzerland)·2025
Same author

Formation of Plant Derived Bioactive Peptides During Simulated Gastro-Intestinal Digestion: A Systematic Review.

BioFactors (Oxford, England)·2025
Same author

Setting Up a "Green" Extraction Protocol for Bioactive Compounds in Buckwheat Husk.

International journal of molecular sciences·2025

Related Experiment Video

Updated: May 5, 2026

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
10:24

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Published on: April 19, 2024

1.7K

ELISA kit for peanut protein determination: collaborative study.

Hana Lexmaulová1, Dana Gabrovská, Jana Rysová

  • 1ELISA Development, Ltd, 412 01 Velk Zernoseky 186, Czech Republic. lexmaulovahana@seznam.cz

Journal of AOAC International
|November 29, 2013
PubMed
Summary

This study validated an enzyme-linked immunosorbent assay (ELISA) for quantifying peanut protein in foods. The validated ELISA method demonstrated reliable detection of peanut protein across various food matrices, establishing clear limits of detection and quantification.

More Related Videos

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

25.5K
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

10.9K

Related Experiment Videos

Last Updated: May 5, 2026

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
10:24

Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus

Published on: April 19, 2024

1.7K
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

25.5K
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

10.9K

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Allergen Detection

Background:

  • Accurate quantification of peanut protein in food is crucial for allergen management.
  • Existing methods may lack specificity or require complex procedures.
  • Development of a reliable ELISA method is essential for food safety regulations.

Purpose of the Study:

  • To collaboratively validate an enzyme-linked immunosorbent assay (ELISA) kit for the quantitative determination of peanut protein in diverse food matrices.
  • To assess the kit's specificity, absence of false-positive results, and cross-reactivity.
  • To establish the method's limits of detection (LOD) and quantification (LOQ).

Main Methods:

  • A collaborative study involving 10 laboratories was conducted.
  • Participants used a standardized ELISA kit based on rabbit polyclonal antibodies.
  • Fifteen food samples, including those with and without declared peanut content, were analyzed.
  • Statistical analysis (Cochran, Grubbs, Mandel, ANOVA) was employed to evaluate results.

Main Results:

  • The ELISA kit showed no false-positive results or cross-reactivity with peanut-free food matrices.
  • Quantitative results for samples with declared peanut content were obtained within the calibration curve in most cases.
  • The collaborative study established a limit of quantification (LOQcollaborative) of 0.22 mg/kg and a limit of detection (LODcollaborative) of 0.07 mg/kg.

Conclusions:

  • The validated ELISA method provides a reliable and specific tool for the quantitative determination of peanut protein in foods.
  • The established LOQ and LOD are suitable for regulatory compliance and allergen control.
  • This collaborative validation supports the widespread adoption of the ELISA kit in food analysis laboratories.