Synthesis and biological properties of two dimeric forms of human alpha-atrial natriuretic peptide

Two dimeric forms of human alpha-atrial natriuretic peptide (alpha-ANP) were synthesized by solution methods and compared with monomeric alpha-ANP in terms of some biological and immunochemical properties. The parallel form (beta'-ANP) and the antiparallel form (beta-ANP) were equipotent in smooth muscle relaxant activity in isolated rat aorta and their ED50 values were estimated to be 1.7 X 10(-8) M and 1.6 X 10(-8) M, respectively. Diuretic and natriuretic responses induced by beta-ANP and beta'-ANP anesthetized rats were equally less potent but exhibited a significantly longer duration than those induced by alpha-ANP. beta-ANP and beta'-ANP possessed immunoreactivities of 60-100% and 50-90% (alpha-ANP, 100% on a molar basis), respectively, with three different antisera raised against alpha-ANP-related peptides.