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Updated: May 5, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Effects of flavonoid-containing preparation Extralife on hydrogen peroxide production and functioning of
S V Murzaeva1, S P Belova, E I Lezhnev
1Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences, Moscow Region; Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia. ldlukyanova@gmail.com.
Abstract:
Extralife, a Pentaphylloides fruticos extract, in concentrations of 0.005-10 μg/ml dose-dependently increased H2O2 production in rat heart mitochondria in the presence of respiration substrates. Extralife decreased ATP-induced accumulation of H2O2 related to inhibition of mitochondrial ATP-dependent potassium channel. This effect was observed only at low doses of the adaptogen (0.05-3 μg/ml). High doses of the substance (5-10 μg/ml) did not abolish ATP-dependent production of H2O2 and increased the rate of H2O2 generation by the mitochondria. We concluded that Extralife in trace concentrations could activate mitochondrial ATP-dependent potassium channel and decrease H2O2 accumulation in the mitochondria.
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