Proteomics study of silver nanoparticles toxicity on Bacillus thuringiensis
Fateme Mirzajani1, Hossein Askari2, Sara Hamzelou2
1Department of Biotechnology, The Faculty of Renewable Energies & New Technologies Engineering (NTE), Shahid Beheshti University, G.C. Evin, Tehran, Iran; Department of Nanobiotechnology, Protein Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran.
Ecotoxicology and Environmental Safety
|December 3, 2013
Summary
Silver nanoparticles (AgNPs) impact Bacillus thuringiensis by altering proteins involved in cell structure and stress response. This proteomic study reveals AgNPs disrupt proton motive force and trigger defense mechanisms in bacteria.
Area of Science:
- Microbiology
- Nanotechnology
- Proteomics
Background:
- Functional genomics and proteomics enable high-throughput analysis of cellular responses to environmental factors.
- Silver nanoparticles (AgNPs) are increasingly studied for their biological effects.
Purpose of the Study:
- To investigate the effects of silver nanoparticles (AgNPs) on Bacillus thuringiensis using a proteomic approach.
- To identify proteins affected by AgNPs exposure and understand the underlying mechanisms.
Main Methods:
- Proteomic analysis using 2-DE (two-dimensional gel electrophoresis) and NanoLC/FT-ICR MS (liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry) for protein identification.
- Exposure of Bacillus thuringiensis to varying concentrations of AgNPs.
Main Results:
- Thirty-four responsive proteins were identified, showing significant up or down-regulation.
- AgNPs exposure led to the accumulation of envelope protein precursors, suggesting dissipation of proton motive force.
- Affected proteins are involved in crucial cellular processes including oxidative stress tolerance, metal detoxification, transcription, protein degradation, and cell division.
Conclusions:
- AgNPs induce significant proteomic changes in Bacillus thuringiensis.
- The observed protein alterations suggest disruption of cellular energy production and activation of defense pathways.
- Understanding these responses is key to evaluating the non-toxicity mechanisms and environmental impact of AgNPs.
