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Updated: Aug 7, 2026

Deep Learning-Based Segmentation of Cryo-Electron Tomograms
Published on: November 11, 2022
Accessible deep learning for automated segmentation of supported nanoparticles in electron microscopy
Christian Vedel Petersen1, Marc Lindgaard1, Nikolaj Nguyen1
1Department of Energy Conversion and Storage, Technical University of Denmark 2800 Kgs. Lyngby Denmark psjq@dtu.dk yanhu@dtu.dk.
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Fast and accurate quantification of the size and morphology of nanoparticles in electron microscopy images is essential for advancing heterogeneous catalysis and energy-conversion research, yet manual segmentation remains the main approach, which is time-consuming, subjective, and challenging to scale. Herein, we present an accessible and efficient deep learning model integrated into a fully functional analysis application for rapid segmentation and statistical quantification. Taken together, this work demonstrates that high-quality nanoparticle segmentation in electron microscopy images is feasible even with minimal annotated data and modest computational resources.
