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Updated: May 5, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
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The Role of PEC Progenitors in ADPKD Progression

Daniele Lodi1, Giulia Ligabue, Valentina Lupo

  • 1Division of Nephrology, Modena Polyclinic, Modena, Italy.

International Journal of Stem Cells
|December 4, 2013
PubMed

Insights

Stem/progenitor cells CD133(+)CD24(+) are involved in Autosomal dominant polycystic kidney disease (ADPKD) progression. These cells are more abundant in ADPKD kidneys, suggesting a role in pathogenesis.

Area of Science:

  • Nephrology
  • Stem Cell Biology
  • Pathogenesis of Kidney Diseases

Background:

  • Autosomal dominant polycystic kidney disease (ADPKD) is characterized by progressive kidney cyst development.
  • Renal tubules normally repair damage using stem/progenitor cells (CD133(+)CD24(+)).
  • Insufficient repair in ADPKD suggests a potential role for these stem cells in disease progression.

Purpose of the Study:

  • To investigate the localization and involvement of CD133(+)CD24(+) cells in ADPKD progression.
  • To determine if these progenitor cells contribute to the pathogenesis of ADPKD.

Main Methods:

  • Analysis of normal and ADPKD kidney tissues.
  • Confocal microscopy and immunoblotting to assess CD133 and CD24 expression.
  • Quantification of CD133(+)CD24(+) cells in renal tissues.

Main Results:

  • CD133 and CD24 expression was observed in a subset of epithelial cells in both normal and ADPKD kidneys.
  • These cells are located in Bowman's capsule and the luminal side of tubules.
  • CD133(+)CD24(+) cells were significantly more represented in ADPKD tubules and healthy glomeruli.
  • ADPKD cysts also expressed CD133 and CD24.

Conclusions:

  • Renal epithelial progenitors (CD133(+)CD24(+)) are involved in ADPKD pathogenesis.
  • Further research is needed to clarify their precise role.
  • Targeting these cells may offer a strategy for disease management and stabilization.
Abstract

Keywords:
ADPKDCD133Damage repairStem cellskidney

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