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Related Experiment Videos

The patch clamp is more useful than anyone had expected.

F J Sigworth

    Federation Proceedings
    |November 1, 1986
    PubMed
    Summary

    The patch clamp technique, developed by Neher and Sakmann, records ionic channel currents in cell membranes. Advances enable its use on cell-free patches and small cells, offering new physiological insights.

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    Area of Science:

    • Biophysics
    • Cell Physiology
    • Electrophysiology

    Background:

    • The patch clamp technique is crucial for studying ion channel function.
    • Neher and Sakmann developed the current form of this technique.
    • Ionic channels are vital for excitable membrane function.

    Purpose of the Study:

    • To summarize the development and applications of the patch clamp technique.
    • To highlight recent advancements in patch clamp methodology.
    • To underscore the technique's broad physiological relevance.

    Main Methods:

    • Utilizing the patch clamp technique for recording ionic currents.
    • Applying the method to single ionic channels.
    • Adapting the technique for cell-free membrane patches and small cells.

    Main Results:

    • Provided new insights into the functioning of individual ionic channels.
    • Demonstrated the adaptability of the patch clamp technique.
    • Shown applicability to diverse physiological problems.

    Conclusions:

    • The patch clamp technique is a versatile tool in biophysics and physiology.
    • Recent modifications have expanded its utility.
    • It continues to yield significant discoveries in channel function and beyond.

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