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In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans
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Hot-spot residues in the cytochrome P450cam-putidaredoxin binding interface

Yoshitaka Hiruma1, Ankur Gupta, Alexander Kloosterman

  • 1Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333 CC Leiden (The Netherlands).

Chembiochem : a European Journal of Chemical Biology
|December 5, 2013
PubMed
Summary

This study investigated polar residues in the Putidaredoxin-Cytochrome P450cam (Pdx-P450cam) complex. Findings reveal these residues are crucial for partner recognition, not electron transfer efficiency.

Keywords:
NMR spectroscopyelectron transferkineticsmutagenesisprotein complexes

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