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Updated: May 5, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
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Editing livestock genomes with site-specific nucleases
Daniel F Carlson1, Wenfang Tan, Perry B Hackett
1Recombinetics Inc., St. Paul, MN 55104, USA.
Reproduction, Fertility, and Development
|December 6, 2013
Summary
Precision genome editing in large animals like pigs and cows has advanced significantly. New tools enable precise genetic modifications for improved livestock, disease models, and biomaterials.
Area of Science:
- Genetics
- Animal Biotechnology
- Molecular Biology
Background:
- Recent advancements in genome editing technologies have revolutionized the ability to precisely manipulate animal genomes.
- Site-specific nucleases offer unprecedented efficiency and accuracy in introducing genetic alterations.
Purpose of the Study:
- To demonstrate the application of site-specific nucleases, particularly transcription activator-like effector nucleases (TALENs), for engineering specific genomic modifications in pigs and cows.
- To explore the potential of precision genetics in livestock improvement and biomedical applications.
Main Methods:
- Utilized site-specific nucleases to create double-strand DNA breaks in large animal genomes.
- Employed non-homologous end joining (NHEJ) for gene inactivation.
- Applied homology-directed repair (HDR) for precise gene alterations, including polypeptide modification, gene elimination, or DNA sequence replacement.
Main Results:
- Achieved effective rates of precise mutations ranging from 10% to 50%, depending on the target gene and mutation type.
- Successfully introduced targeted genetic changes in pigs and cows using TALENs and other site-specific nucleases.
- Demonstrated the versatility of genome editing for various genetic modifications.
Conclusions:
- Precision genome engineering in livestock is now feasible, offering significant potential for enhancing agricultural value and adaptability.
- Engineered animals can serve as valuable models for human diseases, accelerating drug and device development.
- Genetically modified animals hold promise for increased production of essential biomaterials for medical applications.
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