Related Experiment Video
Updated: Jul 9, 2026
Thermal Preconditioning During Ex-vivo Lung Perfusion for the Rehabilitation of Damaged Lung Grafts before Transplantation
Published on: October 31, 2025
Alginate oligosaccharides alleviate heat stress-induced damage to tight junctions and regulate autophagy in sertoli
Wen-Feng Jiang1, Tong Niu1, Zheng-Hui Lan1
1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Context:
Heat stress (HS) induces spermatogenic disorders and reduces sperm quality in males. Alginate oligosaccharides (AOS) exhibit antioxidant and reproductive protective effects, but the molecular mechanism by which AOS improves HS-induced declines in semen quality remains unclear.
Aims:
To investigate the protective effects and underlying molecular mechanism of AOS on HS-induced male reproductive impairment.
Methods:
Six-week-old mice were assigned to the following four groups: control, heat stress, AOS, and AOS + heat stress. We systematically evaluated male fertility, sperm quality, testicular morphology, blood-testis barrier (BTB), tight junctions in Sertoli cells (TJs), and autophagy levels.
Key Results:
The results showed that AOS significantly attenuated the HS-induced reductions in pregnancy rate, litter size, sperm concentration, motility, and acrosome integrity. AOS also alleviated HS-induced testicular structural damage and blood-testis barrier leakage, and restored the expression levels of ZO-1, occludin, and claudin-11. In addition, HS induced abnormally elevated autophagy, as evidenced by increased autophagosomes, upregulated expression of LC3B, Beclin-1, and autophagy-related genes, and decreased p62 expression levels, whereas AOS restored autophagic flux to normal levels.
Conclusions:
The protective effects of AOS relieve heat stress-induced damage to tight junctions via regulating autophagy in Sertoli cells.
Implications:
These findings provide a theoretical basis for AOS as a potential agent for the prevention of HS-related reproductive disorders.
