Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
RNA Interference01:23

RNA Interference

24.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
24.3K
Types of RNA01:23

Types of RNA

61.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
61.3K
Types of RNA01:20

Types of RNA

13.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
13.9K
Types of RNA01:20

Types of RNA

2.0K
2.0K
Types of RNA01:23

Types of RNA

19.7K
19.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Illuminating nanoscale motion using single-molecule Förster resonance energy transfer: Latest insights and innovations.

Current opinion in structural biology·2026
Same author

Quantitative Tissue Proteomics Reveals Protein Signatures Associated with SARS-CoV-2 Variant Infection in Hamsters.

Journal of proteome research·2026
Same author

Disentangling a Complex Biomolecular World with Single-Molecule Resolution.

Chimia·2025
Same author

A genetically based computational drug repurposing framework for rapid identification of candidate compounds: application to COVID-19.

medRxiv : the preprint server for health sciences·2025
Same author

The known unknowns of the Hsp90 chaperone.

eLife·2024
Same author

The potential of fluorogenicity for single molecule FRET and DyeCycling.

QRB discovery·2024

Related Experiment Video

Updated: May 5, 2026

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
11:11

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Published on: December 17, 2016

12.6K

A versatile RNA vector for delivery of coding and noncoding RNAs.

Sonja Schmid1, Lum C Zony, Benjamin R tenOever

  • 1Dept. of Microbiology at the Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Journal of Virology
|December 6, 2013
PubMed
Summary
This summary is machine-generated.

Engineered RNA viruses can deliver therapeutic RNA molecules. A self-replicating, noninfectious RNA platform, based on influenza virus, offers a versatile in vivo system for gene silencing and expression.

More Related Videos

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research
06:41

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research

Published on: October 20, 2023

4.7K
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
12:30

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Published on: October 6, 2017

6.3K

Related Experiment Videos

Last Updated: May 5, 2026

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
11:11

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

Published on: December 17, 2016

12.6K
Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research
06:41

Author Spotlight: Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research

Published on: October 20, 2023

4.7K
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
12:30

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Published on: October 6, 2017

6.3K

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • RNA viruses lack a DNA intermediate, enabling direct RNA manipulation.
  • Engineered RNA viruses present a potential therapeutic delivery platform.
  • Previous research has not fully explored RNA virus-based delivery systems for therapeutic RNA.

Purpose of the Study:

  • To demonstrate the therapeutic potential of engineered RNA viruses as delivery vehicles.
  • To present a self-replicating, noninfectious RNA platform for in vivo RNA delivery.
  • To validate the use of this platform for silencing and/or expressing therapeutic RNAs.

Main Methods:

  • Engineering a self-replicating, noninfectious RNA based on influenza virus.
  • Utilizing the engineered RNA as a delivery system for therapeutic RNAs.
  • Demonstrating in vivo delivery and function of the therapeutic RNA.

Main Results:

  • Successful engineering of a self-replicating, noninfectious RNA platform.
  • Demonstration of the platform's versatility for delivering both silencing and expression RNAs.
  • Validation of in vivo therapeutic RNA delivery and function.

Conclusions:

  • Engineered RNA viruses offer a versatile in vivo delivery system for therapeutic RNA.
  • The developed influenza virus-based RNA platform shows significant therapeutic promise.
  • This platform facilitates RNA-based gene silencing and expression for therapeutic applications.