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Related Experiment Video

Updated: May 5, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Addressable, large-field second harmonic generation microscopy based on 2D acousto-optical deflector and spatial

Yonghong Shao¹, Honghai Liu, Wan Qin

¹College of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.

Applied Physics. B, Lasers and Optics

|December 6, 2013

Summary

We developed a new microscope using a spatial light modulator and acousto-optical deflector for faster, larger field-of-view imaging. This multifocus array scanning technique enhances imaging speed and area compared to traditional methods.

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Area of Science:

Optics and Photonics
Microscopy
Biomedical Imaging

Background:

Second harmonic generation (SHG) microscopy is a valuable tool for label-free imaging.
Traditional SHG microscopy often faces limitations in scanning speed and field-of-view size.
Efficiently scanning multifocal arrays is crucial for advancing SHG imaging capabilities.

Purpose of the Study:

To develop an addressable, large-field second harmonic generation microscope.
To enhance scanning speed and field-of-view size in SHG microscopy.
To enable multi-region imaging through a novel scanning approach.

Main Methods:

Combining a 2D acousto-optical deflector with a spatial light modulator (SLM).
Shaping a Ti:Sapphire laser beam into a multifocus array using the SLM.
Rapidly scanning the multifocus array by altering the laser beam's incident angle with the 2D acousto-optical deflector.

Main Results:

Demonstrated an addressable, large-field SHG microscope.
Achieved rapid scanning of a multifocus array.
Showcased increased scanning rate and field-of-view size compared to single-beam-scan techniques.
Enabled multi-region imaging capabilities.

Conclusions:

The developed multifocus array scanning technique significantly improves SHG microscopy performance.
This approach offers a faster and larger field-of-view alternative to conventional scanning methods.
The system provides enhanced capabilities for advanced biological and materials imaging.