Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

1.0K
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
1.0K
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

1.8K
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
1.8K
Anthelminthic Agents01:15

Anthelminthic Agents

103
Anthelmintic drugs differ significantly from antiparasitic therapies targeting protozoa, primarily due to differences in parasite biology. Whereas most protozoal treatments act on proliferating cells, anthelmintics are typically directed against mature, nonproliferative helminths. The therapeutic approach considers the helminth's reliance on neuromuscular coordination, glucose metabolism, and microtubular integrity for survival, reproduction, and localization within the host. Most anthelmintics...
103
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

828
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
828
Antifungal Agents01:15

Antifungal Agents

119
Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to...
119
Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

130
Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These...
130

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biological Evaluation of Esters of 4-Carboxylate-1,2,3-triazine and Analogs as New Potential Anti-<i>Mycobacterium tuberculosis</i> Agents.

Molecules (Basel, Switzerland)·2026
Same author

<i>In vitro</i> and <i>in vivo</i> activity of omadacycline against <i>Mycobacterium tuberculosis</i>.

Frontiers in microbiology·2026
Same author

Structural Significance and Reference Value of High-Precision Coupling Constants and Raw Data Sharing.

Analytical chemistry·2026
Same author

There is No Caffeine in Damiana-Dismantling Filter Bubbles and Echo Chambers in Scientific Referencing.

Planta medica·2026
Same author

Multilevel Residual Complexity Analysis Reveals Origin of Nanomolar Antiviral Bioactives of "Isoquercitrin".

Journal of natural products·2026
Same author

Long-term nano-dynamic mechanical analysis and micro-permeability of adhesive interface components using four AB-type proanthocyanidin neutral primers.

Journal of dentistry·2026

Related Experiment Video

Updated: May 5, 2026

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
09:34

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Published on: August 16, 2021

2.1K

A novel indigoid anti-tuberculosis agent.

Larry L Klein1, Valentina Petukhova1, Baojie Wan1

  • 1Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 S. Wood Street, Chicago 60612, USA.

Bioorganic & Medicinal Chemistry Letters
|December 10, 2013
PubMed
Summary
This summary is machine-generated.

A novel indigoid compound shows potent anti-tuberculosis activity against Mycobacterium tuberculosis H37Rv. Its structure was determined by X-ray crystallography, and its first synthesis was achieved for further research.

Keywords:
Anti-tuberculosisIndigoidX-ray

More Related Videos

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

8.1K
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
12:17

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Published on: May 8, 2017

11.4K

Related Experiment Videos

Last Updated: May 5, 2026

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
09:34

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Published on: August 16, 2021

2.1K
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

8.1K
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
12:17

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Published on: May 8, 2017

11.4K

Area of Science:

  • Medicinal Chemistry
  • Organic Synthesis
  • Microbiology

Background:

  • Tuberculosis remains a significant global health challenge, necessitating the development of novel therapeutic agents.
  • Indigoid compounds are a class of molecules with diverse biological activities, including potential antimicrobial properties.

Purpose of the Study:

  • To characterize the structure of a novel indigoid component.
  • To evaluate the anti-tuberculosis activity of this compound.
  • To develop a synthetic route for its production.

Main Methods:

  • X-ray crystallography was employed for structural elucidation.
  • Whole cell culture assays were used to determine the minimum inhibitory concentration (MIC) against Mycobacterium tuberculosis H37Rv.
  • Chemical synthesis was performed to obtain sufficient quantities of the compound.

Main Results:

  • The crystal structure of the novel indigoid component was successfully determined.
  • The compound demonstrated significant anti-tuberculosis activity with a submicromolar MIC against Mycobacterium tuberculosis H37Rv.
  • A viable synthetic pathway for the indigoid compound was established.

Conclusions:

  • The characterized indigoid component possesses promising in vitro anti-tubercular activity.
  • This study reports the first synthesis and structural characterization of this novel anti-tuberculosis agent.
  • Further investigation is warranted to explore its therapeutic potential.