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Updated: May 5, 2026

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
An on-demand four-way junction DNAzyme nanoswitch driven by inosine-based partial strand displacement
Alexander H Mo1, Preston B Landon, Brian Meckes
1Materials Science and Engineering Program, University of California, 9500 Gilman Drive, La Jolla, San Diego, CA 92093, USA. plandon@ucsd.edu.
Abstract:
A DNA four-way junction device capable of junction expansion and contraction cycles using an inosine-based partial strand displacement scheme is reported. These nanoscale positioning capabilities are used to provide on-demand activation and deactivation of a pair of split E6 DNAzymes on the device. The device also demonstrates a combined catalytic rate significantly higher than the original E6 DNAzyme under similar operational conditions. This approach can provide structural organization and spatially control other multicomponent molecular complexes.
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