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Related Experiment Video

Updated: May 5, 2026

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
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Cell internalization SELEX: in vitro selection for molecules that internalize into cells.

Amy Yan1, Matthew Levy

  • 1Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 10, 2013
PubMed
Summary

This study presents a method for selecting aptamers that enter cells, not just bind to their surface. This technique enables the recovery of RNA aptamers capable of cellular internalization.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • Aptamer technology utilizes nucleic acid selection for specific molecular binding.
  • Current methods often focus on cell-surface binding, limiting applications requiring cellular entry.

Purpose of the Study:

  • To develop a general scheme for selecting RNA aptamers that internalize into cells.
  • To identify factors influencing the success of aptamer internalization selections.

Main Methods:

  • Selection conditions were established to eliminate non-internalizing and cell-surface binding RNAs.
  • Recovery focused on aptamers with high affinity or those that successfully internalized.

Main Results:

  • A method was described for isolating RNA aptamers capable of cellular internalization.
  • The selection scheme allows for recovery of internalized aptamers or those with extremely tight binding.

Conclusions:

  • This approach expands aptamer selection capabilities beyond cell-surface binding.
  • The described method is adaptable and does not require prior knowledge of cell surface characteristics.