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Related Experiment Video

Updated: May 5, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

DNA electronic switches based on analyte-responsive aptamers.

Jason M Thomas¹, Hua-Zhong Yu, Dipankar Sen

¹Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|December 10, 2013

Summary

This summary is machine-generated.

Researchers developed a novel aptamer-based sensor that acts as a nano-electronic switch. This switch provides an electrochemical readout by controlling DNA charge transfer, enabling sensitive analyte detection.

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Published on: June 1, 2011

Area of Science:

Biotechnology
Nanotechnology
Analytical Chemistry

Background:

Aptamers are highly specific molecular recognition tools.
Analytical sensors require high-affinity and specificity for accurate detection.
Existing sensors can be improved with novel designs for enhanced functionality.

Purpose of the Study:

To develop a generalizable method for creating aptamer-based nano-sized electronic switches.
To enable analyte-responsive electrochemical readout through controlled DNA charge transfer.
To establish biochemical assays for identifying and validating functional aptamer sensors.

Main Methods:

Design of aptamer-modified DNA three-way junctions.
Utilizing through-DNA charge transfer as an electrochemical readout mechanism.
Employing gel electrophoresis-based assays to detect conductivity switching via oxidative DNA damage patterns.

Main Results:

Demonstration of a generalizable process for aptamer-based electronic switch sensors.
Successful implementation of analyte-responsive switching of DNA charge transfer.
Validation of functional sensors using biochemical characterization of DNA damage.

Conclusions:

Aptamer-based nano-electronic switches offer a versatile platform for analytical sensing.
The developed sensors provide a tunable "off" to "on" electrochemical readout.
Biochemical assays are effective for identifying and characterizing these novel biosensing systems.