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Updated: May 5, 2026
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
Published on: February 24, 2017
Adenoviral transduction of multipotent mesenchymal stromal cells from human adipose tissue with bone morphogenetic
T B Bukharova1, L V Logovskaya, A V Volkov
1Medical Genetic Research Center, Russian Academy of Medical Sciences; ReMeTeks Company; N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia. bukharova-rmt@yandex.ru.
Abstract:
We determined conditions for effective transduction of multipotent mesenchymal stromal cells from human adipose tissue with adenoviral constructs carrying the gene of human bone morphogenetic protein BMP-2. The peak of transgene transcription and BMP-2 protein secretion in the transduced cultures was observed on day 6 after infection. The maximum transcription of BMP-2 gene and genes of osteogenic markers (bone sialoprotein, osteopontin, and osteocalcin) was observed in the medium containing sodium β-glycerophosphate and ascorbic acid. Addition of D 3 vitamin did not enhance the expression of BMP-2 gene in transduced cells. The obtained cell cultures with high osteogenic potential can be used in bone tissue engineering.
