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Updated: May 5, 2026
Isolation and Characterization of Microvesicles from Peripheral Blood
Published on: January 6, 2017
Blood microvesicles during chronic lymphoproliferative diseases.
N P Domnikova1, T Yu Dolgikh, E V Sholenberg
1Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences; Research Institute of Molecular Biology and Biophysics, Siberian Division of the Russian Academy of Medical Sciences; Research Institute of Clinical and Experimental Lymphology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia. pathol@soramn.ru.
Patients with B-cell chronic lymphocytic leukemia and indolent non-Hodgkin lymphoma exhibit significantly higher levels of B-cell microvesicles. CD20-positive microvesicle levels correlate with CD20-positive lymphocyte counts in these patients.
Area of Science:
- Hematology
- Oncology
- Immunology
Background:
- Microvesicles, small extracellular vesicles released from cell membranes, play roles in intercellular communication.
- Aberrant microvesicle production is implicated in various hematological malignancies.
- CD19 and CD20 are key surface markers for B-cell lineage and are crucial in B-cell malignancies.
Purpose of the Study:
- To quantify CD19 and CD20-positive microvesicles in patients with B-cell chronic lymphocytic leukemia (CLL) and indolent non-Hodgkin lymphoma (NHL).
- To investigate the correlation between microvesicle levels and lymphocyte counts in chronic lymphoproliferative diseases.
- To explore potential cellular origins of these microvesicles.
Main Methods:
- Flow cytometry was employed to measure the levels of CD19-positive and CD20-positive microvesicles in patient blood samples.
- Comparison of microvesicle levels between patients and healthy volunteers.
- Correlation analysis between microvesicle levels and lymphocyte populations.
Main Results:
- Patients with B-cell CLL and indolent NHL showed significantly elevated levels of B-cell microvesicles compared to healthy volunteers.
- A direct correlation was observed between the level of CD20-positive microvesicles and the number of CD20-positive lymphocytes in patients with chronic lymphoproliferative diseases.
- Extramedullary tumor cells were identified as a potential source of CD19-positive microvesicles.
Conclusions:
- Elevated B-cell microvesicles are a hallmark of B-cell chronic lymphocytic leukemia and indolent non-Hodgkin lymphoma.
- CD20-positive microvesicle levels serve as a potential biomarker reflecting the burden of CD20-positive malignant cells.
- Extramedullary disease may contribute to circulating CD19-positive microvesicles in these malignancies.
