Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

64.7K
Overview
64.7K
Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

3.4K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fumarprotocetraric acid and geraniin were identified as novel inhibitors of human respiratory syncytial virus infection <i>in vitro</i>.

Frontiers in cellular and infection microbiology·2025
Same author

Human adenovirus among hospitalized children with respiratory tract infections in Beijing, China, 2017-2018.

Virology journal·2019
Same author

Anti-H7N9 avian influenza A virus activity of interferon in pseudostratified human airway epithelium cell cultures.

Virology journal·2019
Same author

The generation and biological activity of a long-lasting recombinant human interferon-λ1.

Protein engineering, design & selection : PEDS·2018
Same author

IFN-λ1 in CHO cells: its expression and biological activity.

Cellular & molecular biology letters·2017
Same author

Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR.

Virology journal·2017

Related Experiment Video

Updated: May 5, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

13.6K

[The expression of interferon-lambda1 in CHO cell].

Wu-Mei Yuan1, Fen-Lian Ma, Qian Zhang

  • 1Xinjiang Medical University Pre-clinical College Biochemistry and Molecular Biology Department, China.

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology
|December 11, 2013
PubMed
Summary

Researchers successfully constructed expression vectors for interferon lambda1 and demonstrated its antiviral activity in CHO cells. This work provides a foundation for developing stable interferon lambda1 cell lines.

More Related Videos

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
09:11

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Published on: July 16, 2019

10.6K
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-&#947; Responses
10:10

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Published on: November 16, 2016

12.5K

Related Experiment Videos

Last Updated: May 5, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

13.6K
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
09:11

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Published on: July 16, 2019

10.6K
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-&#947; Responses
10:10

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Published on: November 16, 2016

12.5K

Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • Interferon lambda1 (IFN-λ1) is a cytokine with antiviral properties.
  • Efficient expression systems are needed to produce functional IFN-λ1 for therapeutic applications.

Purpose of the Study:

  • To construct eukaryotic expression vectors linking the SP163 enhancer with interferon lambda1.
  • To express interferon lambda1 in Chinese Hamster Ovary (CHO) dhfr- cells.
  • To evaluate the antiviral activity of the expressed interferon lambda1.

Main Methods:

  • Fusion PCR was used to combine the SP163 enhancer and interferon lambda1.
  • Constructed vectors PCI-dhfr-lambda1 and PCI-dhfr-SP163-lambda1 were confirmed by sequencing.
  • Liposome-mediated transfection introduced vectors into CHO (dhfr-) cells.
  • Protein expression was confirmed by indirect immunofluorescence and Western Blot.
  • Antiviral activity was assessed by inhibition of cytopathic effect.

Main Results:

  • Successfully constructed and sequenced the PCI-dhfr-lambda1 and PCI-dhfr-SP163-lambda1 expression vectors.
  • Demonstrated successful expression of interferon lambda1 in CHO (dhfr-) cells.
  • Confirmed that the SP163 enhancer significantly boosted interferon lambda1 expression.
  • The expressed interferon lambda1 exhibited significant antiviral activity.

Conclusions:

  • Successfully expressed functional interferon lambda1 with antiviral properties in CHO (dhfr-) cells.
  • The SP163 enhancer enhances interferon lambda1 expression.
  • This study provides a valuable basis for establishing stable interferon lambda1-producing cell lines.