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Updated: May 5, 2026
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
Published on: June 19, 2019
Characterization of Lin-ve CD34 and CD117 cell population reveals an increased expression in bone marrow derived stem
Neeru Jindal, Gillipsie Minhas, Sudesh Prabhakar
1Neuroscience Research Lab, Department of Neurology, PGIMER, Chandigarh- 160012, India. akshay1anand@rediffmail.com.
Abstract:
The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear population was separated from rest of the cell population. With the help of Magnetic associated cell sorter (MACS), Linve and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair and regeneration.
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