Related Experiment Video
Updated: May 5, 2026
An Orthotopic Murine Model of Human Prostate Cancer Metastasis
Published on: September 18, 2013
Evaluation of stem cell marker gene expression in canine prostate carcinoma- and prostate cyst-derived cell lines
Mohammed Moulay1, Wen Liu, Saskia Willenbrock
1Prof. Dr., Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30550 Hannover, Germany. ingo.nolte@tiho-hannover.de.
Background/Aim:
In human prostate cancer cells with a stem cell-like character (cancer stem cells, CSC) are considered to play a major role in disease development, progression and relapse. Aim of the study was to evaluate if similar cells are present and active in canine prostate cancer providing a naturally-occurring mammalian model for the development of therapeutic approaches targeting CSC.
Materials And Methods:
Stem cell marker expression of CD133, CD44, C-KIT, CD34, ITGA6, OCT4, DDX5 and MELK in canine prostate carcinomas and prostate cyst cell lines were screened by Polymerase Chain Reaction (PCR), quantitative Polymerase Chain Reaction (qPCR) and partially analysed by flow cytometry.
Results:
Marker analyses by PCR and qPCR, revealed a complex expression pattern for the analysed marker genes, providing a characteristic marker pattern for the studied cell lines. Thereby CD44, CD133, ITGA6 and DDX5 showed the most prominent expression in the analysed cell lines.
Conclusion:
The results revealed a characteristic stem cell marker expression in the analysed cell lines, indicating the presence of CSC in canine prostate cancer.
