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Updated: Sep 6, 2026

14:28
Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
The identification of inhibitors in bromelin one-stage irregular antibody screening techniques
Abstract:
Human sera contain bromelin inhibitors in irregular antibody screening techniques. In order to identify and purify these inhibitors, pooled human sera were precipitated with ammonium sulfate, followed by DEAE-cellulose and Sephadex G-150 column chromatography. Proteinase activity and inhibitory activity to bromelin were observed in two protein fractions, these were identified as alpha 2-macroglobulin and the S-2 thiol proteinase inhibitor.

