Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Voltage gating in porin channels.

J H Lakey

    FEBS Letters
    |January 19, 1987
    PubMed
    Summary

    Porin channel gating in vivo is complex. Differences in purification methods may explain contradictory results regarding voltage gating in E. coli outer membranes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Neural differentiation regulated by biomimetic surfaces presenting motifs of extracellular matrix proteins.

    Journal of biomedical materials research. Part A·2009
    Same author

    Enhanced cell attachment using a novel cell culture surface presenting functional domains from extracellular matrix proteins.

    Cytotechnology·2008
    Same author

    Self-assembling layers created by membrane proteins on gold.

    Biochemical Society transactions·2007
    Same author

    Amphipols: polymeric surfactants for membrane biology research.

    Cellular and molecular life sciences : CMLS·2003
    Same author

    Heat does not come in different colours: entropy-enthalpy compensation, free energy windows, quantum confinement, pressure perturbation calorimetry, solvation and the multiple causes of heat capacity effects in biomolecular interactions.

    Biophysical chemistry·2002
    Same author

    Assembly of pore proteins on gold electrodes.

    Biochemical Society transactions·2001

    Area of Science:

    • Biophysics
    • Molecular Biology
    • Membrane Protein Research

    Background:

    • Porin channels are crucial for the outer membrane of Gram-negative bacteria like E. coli.
    • Understanding porin channel gating is essential for comprehending nutrient transport and antibiotic resistance.
    • Reconstituted porin channels in planar lipid bilayers are a common model system for studying channel function.

    Purpose of the Study:

    • To review experimental data on reconstituted porin channels.
    • To assess the relevance of these findings to porin channel gating in vivo.
    • To discuss the potential for voltage gating in E. coli porins.

    Main Methods:

    • Review of existing experimental data from reconstituted porin channel studies.
    • Analysis of purification techniques and their potential impact on experimental outcomes.
    • Theoretical discussion on the physiological relevance of voltage gating.

    Main Results:

    • Contradictory findings exist regarding voltage-gating properties of porins.
    • Variations in purification methods appear to be a significant factor contributing to conflicting results.
    • The magnitude of the outer membrane potential in E. coli may limit the physiological relevance of voltage gating.

    Conclusions:

    • The gating behavior of porin channels reconstituted in vitro may not directly reflect their in vivo function.
    • Standardization of purification techniques is crucial for reliable porin channel research.
    • Further investigation is needed to definitively establish the role of voltage gating in E. coli porins under physiological conditions.

    Related Experiment Videos