Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hospital-Level Variation in Transcatheter vs Surgical Aortic Valve Replacement Among Patients Younger Than 65 Years.

The Annals of thoracic surgery·2026
Same author

Corrigendum to 'NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis' [Journal of Molecular and Cellular Cardiology 102 (2017) 10-21].

Journal of molecular and cellular cardiology·2026
Same author

Nucleic acid-based therapeutics to restore joint homeostasis in age-related and post-traumatic arthritis.

npj biomedical innovations·2026
Same author

Transforming Postoperative Care: Advanced Recovery Room Care for Intermediate-risk Noncardiac Surgical Patients.

Anesthesiology·2025
Same author

Guidelines for Enhanced Recovery After Trauma and Intensive Care (ERATIC): Enhanced Recovery After Surgery (ERAS) and International Association for Trauma Surgery and Intensive Care (IATSIC) Society Recommendations: Part 3: Trauma Ethics and Systems Aspects.

World journal of surgery·2025
Same author

Guidelines for Enhanced Recovery After Trauma and Intensive Care (ERATIC): Enhanced Recovery After Surgery (ERAS) and International Association for Trauma Surgery and Intensive Care (IATSIC) Society Recommendations: Paper 2: Postoperative and Intensive Care Recommendations.

World journal of surgery·2025

Related Experiment Video

Updated: May 4, 2026

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

28.4K

Maximizing exosome colloidal stability following electroporation.

Joshua L Hood1, Michael J Scott1, Samuel A Wickline1

  • 1Consortium for Translational Research in Advanced Imaging and Nanomedicine (C-TRAIN), Cardiovascular Division, Department of Medicine, Washington University School of Medicine, 4320 Forest Park Avenue, Suite 101, Campus Box 8215, St. Louis, MO 63108, USA.

Analytical Biochemistry
|December 17, 2013
PubMed
Summary

A novel trehalose pulse media (TPM) was developed to stabilize exosomes during electroporation, minimizing aggregation and enabling efficient cargo loading for diagnostic and therapeutic applications.

Keywords:
ElectroporationExosomesIron oxideSuperparamagneticTrehalose

More Related Videos

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

7.8K
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
06:59

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

9.8K

Related Experiment Videos

Last Updated: May 4, 2026

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

28.4K
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

7.8K
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
06:59

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

9.8K

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Materials Science

Background:

  • Exosome-based nanovesicles are promising for diagnostics and therapeutics.
  • Electroporation is a method for loading exosomes with cargo, but can affect colloidal stability.
  • Exosome aggregation post-electroporation has not been adequately studied.

Purpose of the Study:

  • To develop a novel pulse media that minimizes exosome aggregation after electroporation.
  • To evaluate the efficacy of the new media in maintaining exosome colloidal stability and enabling cargo loading.
  • To assess the potential for label-free enrichment and in vivo applications of modified exosomes.

Main Methods:

  • Development of trehalose pulse media (TPM) and comparison with PBS and sucrose pulse media (SPM).
  • Dynamic light scattering (DLS) and RNA absorbance to assess exosome aggregation and electroextraction.
  • Transmission electron microscopy (TEM) to visualize exosome morphology and aggregation.
  • Sucrose gradient ultracentrifugation to evaluate exosome density after cargo loading.

Main Results:

  • TPM significantly minimized exosome aggregation post-electroporation compared to PBS and SPM.
  • Exosome disaggregation in TPM was dependent on exosome concentration and electric field strength.
  • TPM facilitated efficient loading of superparamagnetic iron oxide nanoparticles (SPIONs) into exosomes, maintaining size and stability.
  • Exosome loading with SPIONs increased their density, allowing for label-free enrichment.

Conclusions:

  • Trehalose pulse media (TPM) is an effective formulation for stabilizing exosomes during electroporation.
  • TPM enables efficient cargo loading while preserving exosome integrity.
  • This method offers a label-free approach for exosome enrichment and opens possibilities for MRI-guided theranostic applications.