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An individually addressable suspended-drop electroporation system for high-throughput cell transfection
Youchun Xu1, Ying Lu, Wanli Xing
1Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China. wlxing@tsinghua.edu.cn.
Lab on a Chip
|December 17, 2013
Summary
A novel printed circuit board (PCB)-based electroporation device enables high-throughput, targeted delivery of molecules into cells. This cost-effective technology offers high efficiency and cell viability for various research applications.
Area of Science:
- Biotechnology
- Cell Biology
- Bioengineering
Background:
- Efficient delivery of molecules into cells is crucial for cell biology research.
- Existing methods often lack high-throughput capabilities or cause cell damage.
Purpose of the Study:
- To develop a printed circuit board (PCB)-based electroporation device for high-throughput, individually addressable molecule delivery into cells.
- To achieve high transfection efficiency and cell viability without permanent cell damage.
Main Methods:
- A PCB with 96 through-holes, each featuring gold-coated electrodes, was designed.
- Cells and exogenous molecules were loaded, electroporated, and ejected into a 96-well plate.
- Selective electrode energization allowed for customized electroporation parameters.
Main Results:
- Demonstrated effective introduction of plasmid DNA and synthetic interfering RNA into cultured and primary cells.
- Achieved high cell viability and transfection efficiency.
- The device facilitates individually addressable electroporation for each of the 96 wells.
Conclusions:
- The PCB-based electroporation device offers a high-performance, low-cost solution for cellular and molecular research.
- It is an ideal platform for high-throughput screening and gene/protein/drug delivery applications.
