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Related Experiment Video

Updated: May 4, 2026

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
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NADH changes during hypoxia, ischemia, and increased work differ between isolated heart preparations.

Anastasia M Wengrowski1, Sarah Kuzmiak-Glancy, Rafael Jaimes

  • 1Department of Electrical and Computer Engineering, The George Washington University, Washington, District of Columbia; and.

American Journal of Physiology. Heart and Circulatory Physiology
|December 17, 2013
PubMed
Summary

Isolated heart preparations reveal varying metabolic responses to oxygen levels. Working heart models are most sensitive to oxygen changes, highlighting limitations in studying cardiac energy supply and demand.

Keywords:
Langendorffbiventricular workingblebbistatinnadh imagingworkload

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Area of Science:

  • Cardiovascular Physiology
  • Metabolic Imaging
  • Cardiac Function Studies

Background:

  • Isolated heart preparations like Langendorff-perfused and working hearts are vital for studying cardiac physiology, particularly with fluorescence imaging.
  • These preparations have distinct oxygen and energy demands, crucial for interpreting metabolic data.
  • Nicotinamide adenine dinucleotide (NADH) fluctuations reflect metabolic imbalances, offering insights into tissue energy status.

Purpose of the Study:

  • To compare the metabolic sensitivity to oxygen and workload variations across different isolated rabbit heart preparations.
  • To elucidate the limitations of various isolated heart models concerning energy supply and demand dynamics.
  • To assess the utility of NADH fluorescence imaging in evaluating cardiac metabolic states under physiological stress.

Main Methods:

  • New Zealand white rabbit hearts were utilized, prepared as mechanically silenced Langendorff-perfused, Langendorff-perfused, or biventricular working hearts.
  • Preparations were subjected to altered workload (fast pacing), global ischemia, and gradual hypoxia.
  • Simultaneous monitoring of heart rate, aortic pressure, and epicardial NADH fluorescence was performed.

Main Results:

  • Biventricular working hearts exhibited significant NADH fluctuations with pacing changes, unlike Langendorff preparations.
  • Working hearts showed highest sensitivity to hypoxia, with half-maximal NADH at 67.8% oxygen.
  • Langendorff-perfused and mechanically arrested hearts demonstrated lower sensitivity to reduced oxygen levels (42.5% and 23.7% oxygen, respectively).

Conclusions:

  • NADH imaging effectively tracks metabolic state in isolated heart preparations under varying conditions.
  • Biventricular working hearts, while mimicking in vivo function, display greater metabolic vulnerability to oxygen and workload.
  • Understanding preparation-specific limitations is essential for accurate interpretation of cardiac energy metabolism studies.