Related Experiment Video
Updated: May 4, 2026
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
Published on: June 2, 2022
Complex dynamic substrate control: dual-tone hydrogel photoresists allow double-dissociation of topography and
Changying Xue1, Darice Y Wong, Andrea M Kasko
1Department of Bioengineering, University of California, Los Angeles, 410 Westwood Plaza, 4121 Eng V, Los Angeles, CA, 90095, USA; California Nanosystems Institute, 570 Westwood Plaza, Los Angeles, CA, 90095, USA.
Abstract:
Complex substrate control is demonstrated with a dual-tone hydrogel photoresist. By exposing a photodegradable hydrogel to UV light through a photomask, both swollen and eroded micropatterns with a decreased modulus can be created on the surface under different exposure conditions. This provides an important tool for investigating the synergistic effects of spatially heterogeneous mechanical and topological cues on cell behavior.
