[Use of immunospecific components labelled with beta-lactamase and horseradish peroxidase for immunoenzyme analysis]

A system for enzyme immunoassay with the use of beta-lactamase as an enzyme marker is described. Similar sensitivity of the enzyme immunoassays with the use of beta-lactamase and horse-raddish peroxidase as enzyme markers was shown. The data on decrease of the antigen binding capacity of the antibodies in the conjugates with beta-lactamase are presented. It is suggested that such a decrease may be associated with glutaric dialdehyde conjugation. The use of the schemes for enzyme immunoassay with simultaneous application of two independently recorded enzymes i. e. horse-raddish peroxidase and beta-lactamase provided confirmation of univalency of the conjugate of the melted capsular antigen of the plague causative agent with beta-lactamase. Identity of the antigenic determinants of the melted antigen and capsular antigen of the plague microbe was demonstrated.