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Updated: Aug 6, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
Published on: November 7, 2012
Adaptation of enzymes to temperature: myofibrillar adenosine triphosphatase from two terrestrial isopod species
Abstract:
A comparison of the temperature profiles of the myofibrillar ATPase in Porcellio spinicornis and Metoponorthus pruinosus showed that Porcellio enzyme had a maximum activity between pH 6.8-7.2 at 9 degrees C, and that from Metoponorthus between pH 7.0-7.4 at 45 degrees C. The energy of activation for Porcellio enzyme was estimated to be 2.964 kcal/mol and that for Metoponorthus enzyme 9.91 X 10(-1) kcal/mol. The significance of these findings in relation to the natural habitats of these two species is discussed.
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