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Updated: May 4, 2026
Author Spotlight: Advancing Reproductive Immunology with a Protocol for the Quantitative Evaluation of Endometrial Immune Cells
Published on: October 13, 2023
Comparison of HSCORE assessment of endometrial beta3 integrin subunit expression with digital HSCORE using
Daniele G Fuhrich1, Bruce A Lessey2, Ricardo F Savaris1
1Department of Gynecology and Obstetrics, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Objective:
To compare intraobserver and interobserver variation between traditional histological score (HSCORE) and digital HSCORE (D-HSCORE) performed by expert and naive researchers.
Study Design:
Immunohistochemical analysis of beta3 integrin subunit of 100 endometrial biopsies obtained from the midluteal phase of the menstrual cycle were reanalyzed using ImageJ software (D-HSCORE). Mean intensity of 3,3'-diaminobenzidine on endometrial glands was read by an expert (HSCORE) versus inexperienced observer using HSCORE and D-HSCORE.
Results:
The mean correlation [r(s)(95% CI)] between both methods was 0.86 (0.79-0.90) and highly significant (p < 0.0001) for the experienced individual. The naive researcher overestimated immunostaining, resulting (HSCORE) in negative samples. No discrepancies were seen with D-HSCORE. Interobserver variation for the inexperienced reader was 50% using HSCORE (cutoff 0.7) but 0% with D-HSCORE. Intraobserver variation using ImageJ was 0%.
Conclusion:
The D-HSCORE performed by an inexperienced researcher has high correlation to traditional HSCORE performed by an expert.
