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[Circulating microRNA expression in children with idiopathic short stature]
Sha Zhao1, Yan Zhong, Yao-Hui Jiang
1Department of Child Health Care, Hunan Children's Hospital (Paediatric Academy of University of South China), Changsha 410007, China. yizhuwen@163.com.
Objective:
To study the role of circulating microRNAs (miRNA) in the pathogenesis of idiopathic short stature (ISS) through detecting miRNA expression profile in plasma of children with ISS.
Methods:
Plasma miRNA expression was determined by microarray in 20 children with ISS and 20 healthy children. Altered microRNAs were verified by real-time PCR. The online miRNA target gene prediction software was used to predict and screen miRNA differentially expressed target genes.
Results:
According to the microarray, there were 40 differentially expressed miRNAs in the ISS group compared with the control group, including 24 up-regulated miRNAs and 16 down-regulated miRNAs. Real-time PCR verified two up-regulated (miR-185and miR-574-5p) and two down-regulated miRNAs (miR-497and miR-15a) and confirmed that plasma miR-185 expression was significantly up-regulated (P<0.05) and miR-497 expression was significantly down-regulated (P<0.05) in children with ISS.
Conclusions:
Plasma miRNA expression levels in children with ISS are significantly different from healthy controls, suggesting that plasma miRNA is associated with the pathogenesis of ISS.
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