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Published on: October 6, 2022
Identification and expression profiles of prdm1 in medaka Oryzias latipes
Haobin Zhao1, Xiaosha Zhang, Nana Cheng
1Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, 430079, China, zhaohb@mail.ccnu.edu.cn.
Abstract:
Mouse Prdm1, also known as Blimp1, plays important roles in maturation and survival of lymphoid cells, as well as in organogenesis of muscle, limb, sensor organs and primordial germ cells. The homologues of mouse prdm1 have been identified in a diverse of animals including zebrafish and fugu. Here, we report the identification and expression profiles of two homologues of prdm1, namely prdm1a and prdm1b in medaka, Oryzias latipes. The transcripts of prdm1a and prdm1b were detectable in all the tissues including immune organs such as gill, spleen, kidney, liver and intestine that we have checked on. The transcripts of prdm1a could be detected in the embryonic shield at mid-gastrula stage and later in the somite, eye, otic vesicle, branchial arches, fin, intestine and cloaca during embryogenesis using in situ hybridization. Moreover, the expression of prdm1a in the liver of both medaka and zebrafish could be up-regulated by the immune stimuli including lipopolysaccharide, polyI:C and the grass carp reovirus, similarly to the up-regulation of IL1B. These results indicate that Prdm1a may play important roles in embryogenesis and also in immune response in fish.
Insights
Researchers identified two Prdm1 genes, prdm1a and prdm1b, in medaka fish. Prdm1a is involved in embryonic development and immune responses, showing expression in various tissues and upregulation with immune stimuli.
Area of Science:
- Developmental Biology
- Immunology
- Genetics
Background:
- Mouse Prdm1 (Blimp1) is crucial for lymphoid cell maturation and organogenesis.
- Homologues of Prdm1 exist in various animal species, including fish.
- Understanding Prdm1 function in fish can provide insights into vertebrate development and immunity.
Purpose of the Study:
- To identify and characterize Prdm1 homologues in medaka (Oryzias latipes).
- To investigate the expression patterns of medaka Prdm1 genes during embryogenesis and in adult tissues.
- To explore the role of medaka Prdm1a in immune responses.
Main Methods:
- Identification of medaka prdm1a and prdm1b gene sequences.
- Analysis of gene expression using RT-PCR in various adult tissues.
- In situ hybridization to determine prdm1a expression during embryonic development.
- Immune challenge experiments (LPS, polyI:C, virus) in medaka and zebrafish, followed by gene expression analysis.
Main Results:
- Two medaka Prdm1 homologues, prdm1a and prdm1b, were identified.
- Transcripts for both genes were found in all examined adult tissues, including immune organs.
- prdm1a was detected in embryonic tissues and upregulated in the liver of medaka and zebrafish upon immune stimulation, similar to IL1B.
Conclusions:
- Medaka Prdm1a and Prdm1b are expressed broadly in adult tissues.
- Prdm1a plays a significant role in medaka embryogenesis.
- Prdm1a is likely involved in the immune response pathways in fish.
