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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Lipid bilayer phase transformations detected using microcantilevers.

Jinghui Wang1, Kai-Wei Liu, Laura Segatori

  • 1Department of Chemical and Biomolecular Engineering, Rice University , Houston, Texas 77005, United States.

The Journal of Physical Chemistry. B
|December 19, 2013
PubMed
Summary
This summary is machine-generated.

Microcantilevers detect lipid phase transitions by measuring surface stress changes. This method accurately determines the phase transition temperature (Tm) of lipid bilayers and monolayers, revealing effects of cholesterol and solid supports.

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Area of Science:

  • Biophysics
  • Materials Science
  • Surface Chemistry

Background:

  • Lipid bilayers and monolayers undergo solid-liquid phase transitions.
  • These transitions involve conformational changes and free energy alterations.
  • Surface stress changes accompany phase transitions in supported lipid systems.

Purpose of the Study:

  • To utilize microcantilevers for measuring the phase transition temperature (Tm) of supported lipid bilayers and monolayers.
  • To investigate the influence of solid supports and cholesterol on lipid phase behavior.
  • To assess the stability differences between lipid bilayers and monolayers.

Main Methods:

  • Employing microcantilevers to detect surface stress variations.
  • Monitoring changes in surface stress of 1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC) as a function of temperature.
  • Comparing phase transition temperatures of supported MPPC with free membranes and cholesterol-containing bilayers.

Main Results:

  • Microcantilevers successfully measured the phase transition temperature (Tm) of supported lipid bilayers and monolayers.
  • Surface stress decreased linearly with temperature, with an abrupt jump at Tm.
  • MPPC monolayer Tm was shifted by the solid support, and cholesterol addition lowered Tm by ~0.38 °C per mol %.
  • Sensitive surface stress measurements revealed stability differences between MPPC bilayers and monolayers.

Conclusions:

  • Microcantilever-based surface stress measurements provide a sensitive method for determining lipid phase transition temperatures.
  • The solid support influences the structure and phase behavior of lipid monolayers.
  • Cholesterol incorporation significantly affects lipid bilayer phase transition temperatures and stability.