Related Experiment Video
Updated: Jun 13, 2026
06:21
Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
Published on: May 10, 2024
Integrated Single-Cell Virtual Knockout and Machine Learning Analyses Reveal a Protective Role of CKAP2 in Gastric
Jianhua Yang1, Zheng Qiu1, Wenchao Song1
1School of Medical Informatics Engineering, Anhui University of Chinese Medicine, Hefei 230012, China.
Molecules (Basel, Switzerland)
|June 12, 2026
Summary
Cytoskeleton-associated protein 2 (CKAP2) shows promise as a protective factor and prognostic biomarker in gastric cancer (GC). Elevated CKAP2 expression correlates with better outcomes, potentially via the 5-HT pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gastric cancer (GC) remains a significant global health challenge.
- Identifying novel biomarkers for GC prognosis and treatment is crucial.
Purpose of the Study:
- To investigate the role of cytoskeleton-associated protein 2 (CKAP2) in gastric cancer progression.
- To evaluate CKAP2's prognostic significance and potential protective role.
Main Methods:
- Differential gene expression and WGCNA identified candidate GC genes.
- Machine learning, PPI networks, and single-cell RNA sequencing pinpointed CKAP2 as a key gene.
- Survival analysis, Mendelian randomization, and Western blot validated CKAP2's role and expression.
Main Results:
- CKAP2 was identified as a key gene through integrative analyses.
- Elevated CKAP2 expression was linked to improved GC prognosis.
- CKAP2 may act as a protective factor, potentially through the 5-HT pathway, while 5-HT showed increased GC risk.
Conclusions:
- CKAP2 serves as a potential prognostic biomarker and protective factor in gastric cancer.
- CKAP2's mechanism may involve regulating the 5-HT pathway.
- These findings offer insights into GC pathogenesis and potential therapeutic targets.
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