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Updated: May 4, 2026

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
Published on: March 2, 2018
Neutrophil microparticles modulate cytokine production by natural killer cells
Boris K Pliyev1, Mariya V Kalintseva1, Sofya V Abdulaeva2
1Hematology Research Center, Novy Zykovsky Pr. 4, Moscow 125167, Russia.
Abstract:
Neutrophil microparticles (NMs) are scarce in the circulation but are highly enriched at sites of inflammation and exert profound effects on immune cells. In the present study, we addressed whether NMs modulate cytokine-producing capacity of natural killer (NK) cells. NMs inhibited the production of IFN-γ and TNF-α but enhanced the release of TGF-β1 by IL-2/IL-12-activated NK cells. The inhibitory effect of NMs was strongly attenuated by blockade of phosphatidylserine exposed on NMs. Thus, NMs skew the cytokine profile of NK cells from pro-inflammatory toward anti-inflammatory, potentially favoring the resolution of inflammation.
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