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Cloning and expression of cDNA encoding human basic fibroblast growth factor.

T Kurokawa, R Sasada, M Iwane

    FEBS Letters
    |March 9, 1987
    PubMed
    Summary
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    Researchers isolated a human basic fibroblast growth factor (bFGF) cDNA, revealing multiple mRNA forms and secreted mitogenic activity in transfected cells. This study advances understanding of bFGF gene expression and protein secretion mechanisms.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Basic fibroblast growth factor (bFGF) is a key signaling protein involved in cell growth, differentiation, and wound healing.
    • Understanding the molecular mechanisms of bFGF gene expression and protein secretion is crucial for its therapeutic applications.

    Purpose of the Study:

    • To isolate and characterize the cDNA encoding human basic fibroblast growth factor (bFGF).
    • To investigate the presence of multiple bFGF mRNA species.
    • To assess the secretion and biological activity of the synthesized bFGF protein.

    Main Methods:

    • cDNA library screening using human foreskin fibroblast cells.
    • Northern blot analysis to detect bFGF mRNA species.
    • COS cell transfection to express and analyze bFGF protein activity.

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    Main Results:

    • A 4-kilobase cDNA encoding human bFGF was successfully isolated.
    • At least five distinct bFGF mRNA species were identified in cultured fibroblasts.
    • Transfected COS cells exhibited mitogenic activity in their culture medium, indicating bFGF secretion.

    Conclusions:

    • The human bFGF gene exists in multiple mRNA forms.
    • The synthesized bFGF protein may be secreted from cells, despite a short signal sequence.
    • This research provides insights into bFGF gene regulation and protein processing.