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Updated: Aug 16, 2026

Dissection and Staining of Drosophila Larval Ovaries
Published on: May 13, 2011
[Nucleoli and nucleolus-like bodies in oogenesis stained by silver nitrate]
Abstract:
The nuclear structures were studied in oocytes of two frog species (Rana temporaria and R. ridibunda) in oocytes and trophocytes of three insect species (Blaps lethifera, Laspeyresia pomonella and Chrysopa perla) by the AgNO3-staining method (Howell, Black, 1980). The previously investigated nucleoli of the insect trophocytes and the extra-r-DNA bodies of Chrysopa oocytes were used as test objects. It is demonstrated that in oogenesis, contrary to somatic cells, AgNO3 impregnates both transcriptionally active nucleoli and inactive nuclear structures: extra-r-DNA bodies, nucleolus-like bodies (NLB) and some microbodies. The relationship of Ag+ NLB and microbodies to nucleolar structures is discussed in addition to the role of the r-DNA compactness for the Ag+ reaction.
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