Related Experiment Video
Updated: May 4, 2026
High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
Published on: June 10, 2025
Performance of an automated multiplex immunofluorescence assay for detection of Chlamydia trachomatis immunoglobulin
David Baud1, Jade Zufferey2, Patrick Hohlfeld2
1Center for Research on Intracellular Bacteria, Institute of Microbiology, Faculty of Biology and Medicine, University of Lausanne and University Hospital Center (CHUV), Lausanne, Switzerland; Departments of Obstetrics and Gynecology, Materno-fetal and Obstetrics Research Unit, University Hospital Center (CHUV), Lausanne, Switzerland.
Abstract:
Chlamydia serology is indicated to investigate etiology of miscarriage, infertility, pelvic inflammatory disease, and ectopic pregnancy. Here, we assessed the reliability of a new automated-multiplex immunofluorescence assay (InoDiag test) to detect specific anti-C. trachomatis immunoglobulin G. Considering immunofluorescence assay (IF) as gold standard, InoDiag tests exhibited similar sensitivities (65.5%) but better specificities (95.1%-98%) than enzyme-linked immunosorbent assays (ELISAs). InoDiag tests demonstrated similar or lower cross-reactivity rates when compared to ELISA or IF.
