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Updated: May 4, 2026
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
Expression profiles of 507 proteins from a biotin label-based antibody array in human colorectal cancer
Hisaaki Miyoshi1, Asahiro Morishita1, Joji Tani1
1Department of Gastroenterology and Neurology, Kagawa University Faculty of Medicine, Kita-gun, Kagawa 761-0793, Japan.
Abstract:
Molecular-targeted therapy is one of the most promising therapies for patients with advanced-stage colorectal cancer (CRC). However, a wide range of proteins have unknown expression levels in CRC. The purpose of the present study was to determine the expression levels of various proteins related to colorectal carcinogenesis and cancer development. We examined the expression levels of 507 target proteins using a biotin label-based antibody array in 6 human CRC tissues. We also analyzed the clinicopathological features of CRC patients. In CRC tissues, IL-1α, GRO, Glut5, MIG, ICAM-5, VE-cadherin, uPA and Leptin R were increased when compared to levels in normal colon tissues. MPIF-1/CCL23, FGF R5, MIP2, SAA and IL-18 Rβ were strongly upregulated in rectal cancer when compared to the levels in non-rectal cancer. These data suggest that differential protein expression profiles exist under different conditions, including carcinogenesis and CRC localization. Therefore, an exhaustive analysis of protein expression levels using a biotin label-based antibody protein array is a potentially useful tool for identifying novel individual therapies for CRC patients.
Insights
This study identified key protein expression changes in colorectal cancer (CRC), revealing potential new targets for molecular-targeted therapy in advanced CRC patients.
Area of Science:
- Oncology
- Molecular Biology
- Proteomics
Background:
- Molecular-targeted therapy offers promise for advanced colorectal cancer (CRC).
- Protein expression levels in CRC are not fully understood.
- Identifying novel therapeutic targets is crucial for CRC treatment.
Purpose of the Study:
- To determine protein expression levels in colorectal cancer.
- To identify proteins involved in colorectal carcinogenesis and cancer development.
- To explore potential novel therapeutic targets for CRC.
Main Methods:
- Examined expression levels of 507 target proteins using a biotin label-based antibody array.
- Analyzed protein expression in 6 human colorectal cancer tissues.
- Correlated protein expression with clinicopathological features of CRC patients.
Main Results:
- Increased levels of IL-1α, GRO, Glut5, MIG, ICAM-5, VE-cadherin, uPA, and Leptin R in CRC tissues compared to normal colon tissues.
- Upregulation of MPIF-1/CCL23, FGF R5, MIP2, SAA, and IL-18 Rβ in rectal cancer versus non-rectal cancer.
- Demonstrated differential protein expression profiles in carcinogenesis and CRC localization.
Conclusions:
- Protein expression varies significantly in colorectal cancer based on condition and location.
- Biotin label-based antibody arrays are effective for comprehensive protein analysis in CRC.
- This approach can identify novel therapeutic targets for personalized CRC treatment.
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