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Immunogold Electron Microscopy

Immunogold Electron Microscopy

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Updated: May 4, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

Mining immune epitopes in the inner ear.

Michael Platt¹, Sonam Dilwali, Alphi Elackattu

¹Department of Otolaryngology-Head and Neck Surgery, Boston University School of Medicine, Boston, Massachusetts, USA.

Otolaryngology--Head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery

|December 25, 2013

Summary

This summary is machine-generated.

This study investigated potential immune triggers for inner ear disorders like hearing loss. Researchers found similarities between immune system proteins and inner ear proteins, suggesting a new diagnostic and treatment target.

Keywords:

Meniere’s disease autoimmune inner ear disease bioinformatics computational cross-reactivity epitopes hearing loss inner ear disease

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Last Updated: May 4, 2026

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Cryosectioning and Immunostaining Mouse Inner Ear Tissue: From Embryonic to Adult Stages

Cryosectioning and Immunostaining Mouse Inner Ear Tissue: From Embryonic to Adult Stages

Published on: April 11, 2025

Area of Science:

Immunology
Otolaryngology
Bioinformatics

Background:

The causes of many inner ear disorders, such as autoimmune inner ear disease, sudden sensorineural hearing loss, and Meniere's disease, are largely unknown.
Existing evidence suggests that allergic or autoimmune mechanisms may play a role in these conditions.

Purpose of the Study:

To investigate sequence similarity between known immunogenic proteins and inner ear proteins.
To identify potential cross-reactive epitopes that could trigger detrimental immune responses in the inner ear.

Main Methods:

Comprehensive bioinformatic analysis comparing protein sequences.
Utilized exact match and Basic Local Alignment Search Tool (BLAST) algorithms.
Compared human hearing loss-associated proteins and inner ear proteins against the Immune Epitope Database.

Main Results:

Identified 3036 exact sequence matches and 106 unique epitope matches between immunogenic and inner ear proteins.
The majority of identified matches were associated with infectious epitopes.
This suggests potential targets for immune-mediated inner ear conditions.

Conclusions:

Candidate immune epitopes in the inner ear show sequence similarity to known immunogenic proteins.
These findings may offer novel targets for the diagnosis and treatment of inner ear disorders and hearing loss.
Further clinical validation is required to confirm these potential therapeutic targets.