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[Determination of 2, 4-toluenediamine in urine by gas chromatography]
Wan-chao Zhang1, Bao-feng Liu, Hui Liu
1Tianjin Centers for Disease Control and Prevention, 6th Huayue Street, Tianjin 300011, China.
Objective:
To establish a method for determining the content of 2,4-toluenediamine, a urinary metabolite of toluene diisocyanate, by gas chromatography.
Methods:
Urine samples were collected, and acidification, extraction, derivatization, separation with a capillary column, and detection with an electron capture detector were performed. The target compound was qualified by retention time and quantified by peak area.
Results:
The concentration of 2, 4-toluenediamine showed a linear relationship with peak area within 0.0∼40 ng/ml, with a correlation coefficient 0.9995; the limit of detection was 0.44 ng/ml, the lower limit of quantification was 1.47 ng/ml, the relative standard deviation was 1.85%∼4.05%; the recovery rate was 97.98%∼99.28%.
Conclusion:
The method has the advantages of high sensitivity and high accuracy and can be used for determination of 2, 4-toluenediamine in urine.
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