Cholinergic excitatory synaptic potentials of neurones in mammalian lumbar paravertebral ganglia

Synaptic potentials and the electrophysiological properties of 201 cells in the 4th lumbar paravertebral ganglia of the rabbit were studied in vitro using intracellular electrophysiological recording techniques. Cells had a mean transmembrane potential of 55.1 +/- 0.8 mV, a mean input resistance of 37.0 +/- 6.6 M omega (range 29.9-61.1) and a mean membrane time constant of 6.0 +/- 0.6 ms. Synaptic potentials in ganglionic neurones were evoked by electrical stimulation of the rami communicantes, inferior lumbar splanchnic nerves and the paravertebral chain from segments both above and below the L4 ganglion. Synaptic responses consisted of a fast, hexamethonium-sensitive component and, following short periods of higher frequency stimulation, a slow, long lasting, pirenzepine and atropine-sensitive depolarization (slow-EPSP). No phenomenon corresponding to a late slow-EPSP was observed and, under our recording conditions no cells exhibited non-cholinergic slow excitatory or slow inhibitory postsynaptic potentials. It is concluded that fast excitatory synaptic events were mediated by nicotinic receptors whereas slow excitatory synaptic events were mediated by muscarinic m1 receptors. McNeil-A-343, a muscarinic agonist, produced membrane depolarization, a decrease in membrane input conductance and in some cells a repetitive discharge of action potentials. In 60% of cells tested substance P produced a depolarization of the membrane potential with an associated decrease in membrane input conductance.