Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Positive Regulator Molecules02:39

Positive Regulator Molecules

5.3K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.3K
Positive Regulator Molecules01:45

Positive Regulator Molecules

100.8K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
100.8K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

4.8K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

4.3K
4.3K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.3K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.3K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

2.5K
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemical profile, anti-biofilm and antioxidant activities of Cymbopogon citratus (DC.) Stapf essential oil.

BMC complementary medicine and therapies·2026
Same author

Pleural Fluid Outperforms Plasma for Detection of Clinically Relevant Mutations in Lung Cancer-Associated Malignant Pleural Effusion.

Archivos de bronconeumologia·2026
Same author

Highly dynamic assemblies of glycolytic enzymes by quinary determinants.

Nature communications·2026
Same author

Retraction Note: Zingiber officinale rhizome extracts mediated ni nanoparticles and its promising biomedical and environmental applications.

BMC complementary medicine and therapies·2026
Same author

Retraction notice to "Innovative challenge for the inhibition of hepatocellular carcinoma progression by combined targeting of HSP90 and STAT3/HIF-1α signaling" [Biomedicine & Pharmacotherapy 158 (2023) 114196].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

The Emerging Threat of Monkeypox: An Updated Overview.

Viruses·2026

Related Experiment Video

Updated: May 4, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.3K

A Whi7-anchored loop controls the G1 Cdk-cyclin complex at start.

Galal Yahya1, Eva Parisi1, Alba Flores1

  • 1Molecular Biology Institute of Barcelona (IBMB-CSIC), 08028 Barcelona, Catalonia, Spain.

Molecular Cell
|December 31, 2013
PubMed
Summary

Budding yeast cells enter the cell cycle via a positive feedback loop involving Cln3 cyclin and Whi7. This mechanism ensures cell cycle entry only after reaching a critical size, regulating cell division.

More Related Videos

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

26.6K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

13.7K

Related Experiment Videos

Last Updated: May 4, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.3K
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

26.6K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

13.7K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell cycle commitment at Start involves G1 cyclin-dependent kinase (Cdk) activation, initiating the G1/S transition.
  • In budding yeast, the Cdc28-Cln3 complex is regulated by endoplasmic reticulum (ER) retention for cell size control.

Purpose of the Study:

  • To investigate the molecular mechanisms controlling cell cycle entry and cell size in budding yeast.
  • To identify key regulators of the Cdc28-Cln3 complex and its role in cell cycle commitment.

Main Methods:

  • Isolation and characterization of small-cell-size CDC28 mutants with impaired ER retention.
  • Differential interactome analysis of a quintuple Cdc28(wee) mutant.
  • Investigating the phosphorylation-dependent association of Whi7 with the ER.

Main Results:

  • Small-cell-size CDC28 mutants exhibited premature Cln3 cyclin nuclear accumulation due to impaired ER retention.
  • Whi7, a Whi5 paralog, was identified as a Cdc28 target associated with the ER.
  • Cln3 cyclin and Whi7 form a positive feedback loop that releases the G1 Cdk-cyclin complex.

Conclusions:

  • A novel nonlinear mechanism involving Cln3 cyclin and Whi7 regulates cell cycle entry at Start.
  • This feedback loop ensures cell cycle commitment only upon reaching a critical cell size.
  • The findings uncover key regulatory events at the earliest stages of cell-cycle entry.