Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

587
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
587
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.2K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.2K
Stringent Response in E. coli01:23

Stringent Response in E. coli

528
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
528
Translational Regulation01:29

Translational Regulation

877
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
877
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K
The Unfolded Protein Response01:37

The Unfolded Protein Response

5.6K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The chain mediating roles of emotional resilience and posttraumatic growth in the relationship between perceived stress and cognitive flexibility among military recruits.

Scientific reports·2026
Same author

Correction: Zinc oxide nanoparticle chelated phosphocreatine-grafted chitosan composite hydrogels for enhancing osteogenesis and angiogenesis in bone regeneration.

Frontiers in medicine·2026
Same author

Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

The Journal of cell biology·2026
Same author

Fe-MXene Nanozyme for Dual-Mode Colorimetric-SERS Sensing of Glucose With Mechanistic Insights Into Catalysis and Signal Enhancement.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Development and Validation of a Novel Clinical Prediction Model for Postherpetic Neuralgia: Integrating Inflammatory and Coagulation Biomarkers.

Pain physician·2026
Same author

Type II tRNA cleavage by SLFN14 endoribonuclease variants linked to inherited thrombocytopenia drives global translational repression.

PLoS biology·2026
Same journal

Molecular Interplay of PARN and Telomerase: Tail Modifiers and Disease Implications.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Exploring New Frontiers in Bone Metabolism: Role and Potential of lncRNA DANCR.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Functional Inclusion of RNA Biology in the Tethered Extracellular Matrix.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Structural and Functional Diversity of RNA-Containing Toxin-Antitoxin Systems.

Wiley interdisciplinary reviews. RNA·2026
Same journal

Promoter-Targeting RNA Technologies: An Epigenetic Strategy for Gene Activation and Gene Silencing.

Wiley interdisciplinary reviews. RNA·2026
Same journal

LncRNA PCAT18: Roles and Mechanisms in Human Cancers.

Wiley interdisciplinary reviews. RNA·2026
See all related articles

Related Experiment Video

Updated: May 4, 2026

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

4.1K

Translational reprogramming in cellular stress response.

Botao Liu1, Shu-Bing Qian

  • 1Graduate Field of Genetics, Genomics, and Development, Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA.

Wiley Interdisciplinary Reviews. RNA
|December 31, 2013
PubMed
Summary
This summary is machine-generated.

Cell survival depends on regulating gene expression, particularly messenger RNA (mRNA) translation. Stress responses involve selective mRNA translation, revealing new insights into cell recovery and disease therapies.

More Related Videos

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
10:59

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Published on: May 19, 2014

17.8K
Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
08:39

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

1.9K

Related Experiment Videos

Last Updated: May 4, 2026

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

4.1K
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
10:59

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Published on: May 19, 2014

17.8K
Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
08:39

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

1.9K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cell survival in dynamic environments necessitates precise gene expression regulation, including translational control.
  • Stress signaling pathways converge on translation factors (e.g., eIF4F, eIF2) to modulate mRNA translation during initiation and elongation.
  • Global protein synthesis repression often coincides with selective translation of survival-related mRNAs.

Purpose of the Study:

  • To explore the significant advancements in understanding translational reprogramming over the past decade.
  • To investigate the role of emerging technologies in dissecting mRNA-protein interactions.
  • To address questions raised by genome-wide studies on stress-induced translational reprogramming.

Main Methods:

  • Utilizing ribosome profiling for genome-wide analysis of translation.
  • Examining mRNA elements and their interacting proteins.
  • Investigating alternative translation initiation and elongation dynamics.

Main Results:

  • Genome-wide studies revealed extensive proteome complexity and flexibility via alternative translation.
  • Significant findings include widespread alternative translation initiation and ribosome pausing.
  • Reversible mRNA modifications were also identified as key regulatory mechanisms.

Conclusions:

  • Translational reprogramming is a critical mechanism for cell survival and stress adaptation.
  • Advanced technologies have illuminated complex regulatory events in mRNA translation.
  • Understanding these mechanisms offers potential for novel therapeutic strategies in human diseases.