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MicroRNA-155 promotes glioma cell proliferation via the regulation of MXI1

Jianwen Zhou1, Wei Wang2, Zhenhua Gao3

  • 1State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China ; Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou, China ; Department of Pathology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Plos One
|December 31, 2013
PubMed

Insights

MicroRNA-155 (miR-155) targets Max interactor-1 (MXI1) in glioma, reducing its expression and promoting tumor cell proliferation. This study reveals a novel mechanism in glioma-genesis involving miR-155 and MXI1.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Gliomas are aggressive primary central nervous system tumors.
  • Max interactor-1 (MXI1), a c-Myc antagonist, is deregulated in various tumors, including glioma.
  • The precise mechanism of MXI1 deregulation in gliomas is not fully understood.

Purpose of the Study:

  • To elucidate the mechanism of MXI1 deregulation in glioma.
  • To identify microRNAs (miRNAs) regulating MXI1 expression in glioma cells.
  • To investigate the role of miR-155 in glioma progression by targeting MXI1.

Main Methods:

  • Bioinformatic analysis and experimental validation to identify regulatory miRNAs.
  • Luciferase reporter assays to confirm direct targeting of MXI1 3'UTR by miR-155.
  • Cell proliferation assays (MTT, EdU, cell counting) and rescue experiments in glioma cell lines.
  • Correlation analysis of miR-155 and MXI1 mRNA levels in glioblastoma specimens.

Main Results:

  • MXI1 expression is significantly down-regulated in glioma cell lines.
  • miR-155 was identified as a potent inhibitor of MXI1, reducing its mRNA and protein levels in U87 cells.
  • miR-155 promotes glioma cell proliferation by targeting MXI1, confirmed through rescue experiments.
  • Inverse correlation observed between MXI1 mRNA and miR-155 expression in glioblastoma samples.

Conclusions:

  • miR-155 directly targets MXI1, leading to decreased MXI1 expression and enhanced glioma cell proliferation.
  • This study uncovers a novel role for miR-155 in glioma-genesis through the suppression of MXI1.
  • Targeting the miR-155/MXI1 axis presents a potential therapeutic strategy for gliomas.

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