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[Study on the chemical composition in different parts of Xanthii Fructus based on UPLC-TOF/MS]
Shu-Min Liu1, Min Cao2, Bin Wu2
1Institute of Traditional Chinese Medicine, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China. keji-liu@163.com
Objective:
To analyze the chemical composition in Xanthii Fructus by UPLC-ESI-TOF/MS.
Methods:
UPLC-ESI-TOF/ MS was used to analyze the samples in positive and negative mode, Masslynx V4.1 workstation was used to analyze data. Through calculating accuracy mass and isotopic fit value of the molecular ion cluster, molecular formula was confirmed.
Results:
33 peaks were detected from the water extract of Xanthii Fructus, 11 peaks were identified by reference substance and elemental composition analysis. 22 peaks were from Xanthim thorn, 31 peaks were from the fruit without stab, 20 of them were identical.
Conclusion:
This method is convenient, accurate and specific, and can be used to find out which parts the component belong to in Xanthium. This study can provide scientific evidence to distinguish the toxic component of Xanthii Fructus and guide clinical medication safety.
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