[Changes in protein in bile excretion from rat liver inoculated with BERH-2 hepatoma]

Rocket immunoelectrophoresis was used to assay the change in alpha fetoprotein (AFP) and albumin (Alb) in bile of rats 10 to 14 days after intrahepatic or intraperitoneal inoculation of BERH-2 hepatoma. The bile was collected from the common bile duct cannula. Total protein was determined by the Lowry method. The results showed that the excretion of total protein and Alb was reduced to 54% and 24% of the control in the intrahepatic group, and 65% and 56% of the control in the intraperitoneal group. Bile-AFP can be detected in rats in both groups and its concentration increased gradually during the tumor progression. In the hepatic group, the bile-AFP content per gram of tumor tissue was 29% of that in the intraperitoneal group. It suggests that decreased excretion of AFP, Alb and total protein in bile of rat bearing tumor in the liver be related to local obstruction of excretion by the tumor mass leading to constriction of biliary canaliculi.