Related Experiment Video

Updated: May 4, 2026

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

Emerging therapies targeting the ubiquitin proteasome system in cancer

Nathaniel M Weathington, Rama K Mallampalli

    The Journal of Clinical Investigation
    |January 3, 2014
    PubMed

    Insights

    The ubiquitin proteasome system (UPS) regulates cellular proteins. UPS-targeting drugs are effective against multiple myeloma and are being developed for other cancers.

    Area of Science:

    • Biochemistry
    • Cellular Biology
    • Pharmacology

    Background:

    • The ubiquitin proteasome system (UPS) is crucial for cellular protein regulation.
    • Proteasome inhibition is a successful strategy for treating multiple myeloma.
    • Emerging UPS-targeting drugs show potential in oncology.

    Purpose of the Study:

    • To review the biology of UPS-targeting drugs.
    • To discuss their application in treating neoplasia.
    • To outline the current development status of novel UPS therapeutics.

    Main Methods:

    • Literature review of UPS biology and drug development.
    • Analysis of clinical and preclinical data for UPS-targeting agents.
    • Discussion of therapeutic strategies involving UPS modulation.

    Main Results:

    • UPS inhibition is effective in multiple myeloma and other cancers.
    • New drugs targeting the UPS are under development as antiproliferatives and immunomodulators.
    • These agents target the proteasome or associated pathways.

    Conclusions:

    • UPS-targeting drugs represent a significant therapeutic avenue for cancer treatment.
    • Ongoing research is expanding the application of UPS inhibitors to various malignancies.
    • Further clinical and preclinical development is crucial for novel UPS-based therapies.

    More Related Videos

    Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model09:00

    Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model

    Published on: April 17, 2026

    332
    Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations10:26

    Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

    Published on: November 7, 2019

    5.1K

    Related Concept Videos

    The Proteasome01:13

    The Proteasome

    Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
    In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
    1.6K
    The Proteasome02:18

    The Proteasome

    Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
    In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
    7.8K
    Targeted Cancer Therapies02:57

    Targeted Cancer Therapies

    The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
    There are several types of targeted therapies against...
    7.0K
    Targeted Cancer Therapies02:57

    Targeted Cancer Therapies

    1.4K
    Regulated Protein Degradation02:58

    Regulated Protein Degradation

    It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
    Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
    6.6K
    The Proteasome Structure01:17

    The Proteasome Structure

    The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
    The proteasome is an...
    2.2K